Journal: PLoS ONE

Article Title: Analysis of Intracellular State Based on Controlled 3D Nanostructures Mediated Surface Enhanced Raman Scattering

doi: 10.1371/journal.pone.0015836

Figure Lengend Snippet: ( A ) SERS spectra of ( 1 ) HeLa, ( 2 ) HepG2, and ( 3 ) MCF-7 cells. ( B ) SERS spectra of (blue) MCF-7 and (black) HMEC cells. ( C ) SERS spectra of living HeLa cells (black curve) and dead HeLa cells (blue curve). Inset contains the confocal microscopic images of living and dead HeLa cells. ( D ) SERS spectra of living HepG2 cells (black curve) and dead HepG2 cells (blue curve). Inset contains the confocal microscopic images of living and dead HepG2 cells. ( E ) SERS spectra of living HMEC cells (black curve) and dead HMEC cells (blue curve). Inset contains the confocal microscopic images of living and dead HMEC cells. Circles in Figs. C, D, and E indicate the primary differences between the SERS spectra of living and dead cells.

Article Snippet: Human liver carcinoma cell line (HepG2), human epithelial carcinoma cell line (HeLa) and human breast adenocarcinoma cell line (MCF-7) were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques:

Journal: Molecules

Article Title: Synthesis and Pharmacological Effects of Diosgenin–Betulinic Acid Conjugates

doi: 10.3390/molecules25153546

Figure Lengend Snippet: Cytotoxicity screening tests (IC 50 values (μM), 72 h).

Article Snippet: The compounds 1 – 8 were subjected to the cytotoxicity screening tests on cells of human T-lymphoblastic leukemia (CEM), cells of human breast adenocarcinoma (MCF7), cells of human cervical cancer (HeLa), and human colon carcinoma (HCT 116 cancer cell lines), using normal human fibroblasts (BJ) as reference cell lines.

Techniques:

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: Plots of expected enhancement rates in DNA dsb in HeLa cells (A) × (C) shown in Table vs enhancement rates in cytotoxicity against HeLa cells (B) for 1 P1–3 (red line) and 1M 1–3 (purple line). The slopes of the red and blue lines are 0.44 and 0.053, respectively.

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques:

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: Confocal microscopic images of 1 P1–3 (200 μM) in HeLa cells in the dark on 1 h incubation. Bright-field images (A, E, I). Blue fluorescence indicates the fluorescence of 1 P1 (B), 1 P2 (F), and 1 P3 (J) (λ ex = 405 nm). Red fluorescence is mitochondrial staining by Mito Tracker Deep Red FM (50 nM) (Thermo Fisher) (C, G, K) (λ ex = 640 nm). (D, H, L) Overlay images of panels (A)–(C), (E)–(G), and (I)–(K), respectively. The scale bar is 20 μm.

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques: Incubation, Fluorescence, Staining

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: In Vitro Cytotoxicity of 1 , 1 X , and Cisplatin against Various Cells in MTT Assay (48 h) (Mean ± SD)

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques: In Vitro, MTT Assay

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: Data for the Plot of Expected Enhancement Rates ( 1 X / 1 ) in DNA dsb in HeLa Cells (A) × (C) vs Enhancement Rates ( 1 X / 1 ) in Cytotoxicity against HeLa Cells (B) a

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques:

Journal: Cancer Cell International

Article Title: Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells

doi: 10.1186/s12935-016-0379-1

Figure Lengend Snippet: Forced expression of TFF3 in cervical cancer cells modulates the expression of malignant progression-related gene markers. a Determination of endogenous expression of TFF3 protein by Western blot in the cervical cancer cells lines CaSki, SiHa, Me180 and Hela and human non-tumor keratinocyte line HaCaT. b Semi-quantitative RT-PCR analysis was used to assess the mRNA levels of TFF3 in SiHa and Hela cells with either forced or depleted expression of TFF3 as described in “ ” section. c Western blot analysis was used to assess the protein levels of TFF3 in SiHa and Hela cells with either forced or depleted expression of TFF3. d , e Quantitative PCR analysis quantifying the change in expression of various genes associated with malignant progression in SiHa-TFF3/Hela cells. Change in gene expression is expressed as fold difference, respectively. Fold change values are representative of three independent experiments

Article Snippet: Human cervical cancer cell lines SiHa, CaSki, Hela, Me180 and human non-tumor keratinocyte line HaCaT were obtained from Nanjing KeyGen Biotech Co, Ltd (Nanjing,China).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Gene Expression